anti rat cd11b pacific blue  (Bio-Rad)

Journal: Cells

Article Title: Sex Dependent Disparities in the Central Innate Immune Response after Moderate Spinal Cord Contusion in Rat

doi: 10.3390/cells13070645

Figure Lengend Snippet: Primary antibodies used for flow cytometry and immunohistochemistry.

Article Snippet: Anti-Rat CD11b:Pacific Blue , Mouse , Bio-Rad (Hercules, CA, USA) , MCA275PB , 1:50.

Techniques: Flow Cytometry, Immunohistochemistry

Journal: bioRxiv

Article Title: Interstitial pneumonia via the oropharyngeal route of infection with Encephalitozoon cuniculi Oropharyngeal infection with Encephalitozoon cuniculi

doi: 10.1101/2024.04.04.588046

Figure Lengend Snippet: Macrophage populations in the lungs or bronchoalveolar lavage (BAL) of the mice infected (Infected group) or not infected (Control group) with E. cuniculi spores. A) Percentage of total macrophages (F4/80 + ), interstitial macrophages (F4/80 + , CD11b + , and SiglecF - ), and alveolar macrophages (F4/80 + , CD11b - , and SiglecF + ) in the lungs of mice. B) Percentage of total macrophages (F4/80 + ), interstitial macrophages (F4/80 + , CD11b + , and SiglecF - ), and alveolar macrophages (F4/80 + , CD11b - , and SiglecF + ) in BAL. The data presented are means ±standard errors of the means (SEMs) (*p < 0.05, **p < 0.01, T-test).

Article Snippet: Afterward, the cells were washed and incubated with the following monoclonal antibodies: peridinin chlorophyll (PerCP)-conjugated or allophycocyanin (APC)-conjugated rat anti-mouse CD19, Phycoerithrin (PE)-conjugated rat anti-mouse CD23, Peridinin Chlorophyll Protein Complex (PerCP)-conjugated rat anti-mouse CD4, FITC-conjugated rat anti-mouse CD8, PE Cy7-conjugated rat anti-mouse F4/80, and pacific blue - conjugated rat anti-mouse CD11b (BD-Pharmingen, San Diego, CA).

Techniques: Infection

Journal: bioRxiv

Article Title: Interstitial pneumonia via the oropharyngeal route of infection with Encephalitozoon cuniculi Oropharyngeal infection with Encephalitozoon cuniculi

doi: 10.1101/2024.04.04.588046

Figure Lengend Snippet: Macrophage populations in the lungs or bronchoalveolar lavage (BAL) of the mice immunosuppressed with cyclophosphamide (Cy) and then infected (Cy-Infected group) or not infected (Cy-Uninfected group) with E. cuniculi spores. A) Percentage of total macrophages (F4/80 + ), interstitial macrophages (F4/80 + , CD11b + , and SiglecF - ), and alveolar macrophages (F4/80 + , CD11b - , and SiglecF + ) in the lungs of mice. B) Percentage of total macrophages (F4/80 + ), interstitial macrophages (F4/80 + , CD11b + , and SiglecF - ), and alveolar macrophages (F4/80 + , CD11b - , and SiglecF + ) in BAL. The data presented are means ±standard errors of the mean (SEMs) (*p < 0.05, **p < 0.01, T-test).

Article Snippet: Afterward, the cells were washed and incubated with the following monoclonal antibodies: peridinin chlorophyll (PerCP)-conjugated or allophycocyanin (APC)-conjugated rat anti-mouse CD19, Phycoerithrin (PE)-conjugated rat anti-mouse CD23, Peridinin Chlorophyll Protein Complex (PerCP)-conjugated rat anti-mouse CD4, FITC-conjugated rat anti-mouse CD8, PE Cy7-conjugated rat anti-mouse F4/80, and pacific blue - conjugated rat anti-mouse CD11b (BD-Pharmingen, San Diego, CA).

Techniques: Infection

Journal: Cancer Research Communications

Article Title: Nanoparticle STING Agonist Reprograms the Bone Marrow to an Antitumor Phenotype and Protects Against Bone Destruction

doi: 10.1158/2767-9764.CRC-22-0180

Figure Lengend Snippet: Effects of STING-NPs on BM immune populations. A, Flow cytometry analysis of BM aspirates from tumor-bearing hindlimbs of STING-NP–treated and untreated mice at days 3, 7, and 14 ( n = 6). Granulocytes (Gran: CD11b + CD11c − Ly6G + ), classical monocytes (cMo: CD11b + Ly6C hi CD11c − Ly6G − ), nonclassical monocytes (ncMo: CD11b + Ly6C lo CD11c − Ly6G − ), and CD11c + Cells (CD11c + CD11b + Ly6C +/− Ly6G +/− ) were quantified as a percentage of CD11B + CD3 − cells. B, Flow cytometry quantification of T cells in tumor-bearing hindlimbs at days 7 and 14 ( n = 3). CD69 + and CD279 + CD8 + or CD4 + cells are reported as a percentage of total T cells (CD45 + CD3 + ). C, Tregs (CD4 + CD25 + FOXP3 + ) cells were measured through flow cytometry at days 7 and 14 ( n = 4) and reported as a percentage of total CD4 + cells. Multiple t tests with Bonferroni correction. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Error bars: SEM.

Article Snippet: A total of 100 μL of cell suspension for each flow test was transferred into a 96-well plate, blocked with BD Fc Block, and stained with a Bio-Rad Murine Myeloid Cell No Compensation Flow Panel: Pacific Blue- CD11b (clone: 5C6, Bio-Rad, catalog no. MCA711PB, RRID:AB_2927543), RPE-Alexa Fluor 750- CD11c (clone: N418, Bio-Rad, catalog no. MCA1369P750, RRID:AB_566465), Alexa Fluor 647- Ly6C (clone: ER-MP20, Bio-Rad, catalog no. MCA2389A647, RRID:AB_2137341), and FITC- Ly6G (clone: 1A8, Bio-Rad, catalog no. MCA6077F, RRID:AB_2927491) .

Techniques: Flow Cytometry

Journal: Cell reports

Article Title: Differential Etv2 threshold requirement for endothelial and erythropoietic development

doi: 10.1016/j.celrep.2022.110881

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rat anti-mouse CD11b-Pacific Blue , Thermo Fisher , RRID: AB_10372795.

Techniques: RNA Sequencing, ChIP-sequencing, Software

Journal: Cell reports

Article Title: Differential Etv2 threshold requirement for endothelial and erythropoietic development

doi: 10.1016/j.celrep.2022.110881

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Single cell suspensions were then stained with a 1:100 dilution of Rat anti-mouse Ter119-PerCP or with a mix of rat anti-mouse CD45-PercP, rat anti-mouse CD11b-Pacific Blue, and rat anti-mouse CD93 at a 1:20 dilution (see ) for 45 min at 4°C with agitation and were then subjected to either flow cytometric analyses on a BD FACS Verse or BD FACS Aria III flow cytometer (BD Biosciences).

Techniques: RNA Sequencing Assay, ChIP-sequencing, Software

Journal: Acta neuropathologica communications

Article Title: Humanized tau antibodies promote tau uptake by human microglia without any increase of inflammation.

doi: 10.1186/s40478-020-00948-z

Figure Lengend Snippet: Fig. 1 Mouse neonatal primary microglia express microglia-specific markers in culture. a Representative fluorescent photomicrographs showing cell morphology of cultured mouse neonatal primary microglia cells. Green, ionizing calcium-binding adaptor molecule 1 (Iba-1), and blue, DAPI nuclear staining. Scale bars, 50 μm. b Flow cytometry analysis confirmed purity of primary microglia culture. Histogram shows the binding for anti-CD11b compared to the binding for nonspecific IgG. Quantification of four independent microglia cultures confirmed that around 95% of cells were positive for microglia marker CD11b (mean +/−SEM). c Analysis of FcγRs confirmed that almost all microglia express FcγI receptor (CD64), FcγII and FcγIII receptor (CD16/32) (mean +/−SEM)

Article Snippet: Monoclonal mouse antibodies DC8E8 and DC25 [23], DC51 [32], DC190 (mapping tau epitope 368–376, Axon Neuroscience SE), rabbit anti-Iba1 (WAKO), blocking antibodies anti-CD16 +CD32 (Abcam) and anti-CD64 (SantaCruz Biotechnologies), rat anti-mouse CD11b Pacific Blue (Biorad), mouse IgG2b isotype control Pacific Blue (Biorad), PE rat anti-mouse CD16/CD32 (BD PharmingenTM), PE mouse IgG2b isotype control (BD PharmingenTM), mouse FcgRIA/CD64a (A594) (R&D systems), and mouse IgG2a isotype control (A594) were used for tau analysis and for mouse microglia.

Techniques: Cell Culture, Binding Assay, Staining, Flow Cytometry, Marker

Journal: Acta neuropathologica communications

Article Title: Humanized tau antibodies promote tau uptake by human microglia without any increase of inflammation.

doi: 10.1186/s40478-020-00948-z

Figure Lengend Snippet: Fig. 4 Human adult primary microglia express specific microglial markers. a Representative fluorescent photomicrographs of human primary microglia culture demonstrating cell morphology and purity of culture; Iba-1 (green), DAPI nuclear staining (blue). Scale bar represents 50 μm. b Flow cytometry analysis for CD11b, CD16, CD32 and CD64 confirmed purity of human microglia cultures. For each marker representative histograms are shown from one human microglia culture and bar graphs with percentage of positive cells from four independent microglia cultures (mean +/−SEM). Histograms and bar graphs show binding and cell positivity for anti-CD11b, −CD16, −CD32 and -CD64, respectively, compared to binding and cell positivity for nonspecific IgG

Article Snippet: Monoclonal mouse antibodies DC8E8 and DC25 [23], DC51 [32], DC190 (mapping tau epitope 368–376, Axon Neuroscience SE), rabbit anti-Iba1 (WAKO), blocking antibodies anti-CD16 +CD32 (Abcam) and anti-CD64 (SantaCruz Biotechnologies), rat anti-mouse CD11b Pacific Blue (Biorad), mouse IgG2b isotype control Pacific Blue (Biorad), PE rat anti-mouse CD16/CD32 (BD PharmingenTM), PE mouse IgG2b isotype control (BD PharmingenTM), mouse FcgRIA/CD64a (A594) (R&D systems), and mouse IgG2a isotype control (A594) were used for tau analysis and for mouse microglia.

Techniques: Staining, Flow Cytometry, Marker, Binding Assay

Journal: Journal of Neuroinflammation

Article Title: Sustained interleukin-10 delivery reduces inflammation and improves motor function after spinal cord injury

doi: 10.1186/s12974-019-1479-3

Figure Lengend Snippet: Flow cytometry performed seven days post-injury. Activated macrophages were distinguished by their expression of CD11b and CD45 ( a ). No difference was observed in the number of CD11b + CD45 HIGH macrophages between treatment groups ( b ). * P < 0.05 (Tukey’s Test); error bars represent ± SEM; n = 3 for Controls and 4 for all other groups

Article Snippet: The cells were then incubated in 100 μL of primary antibody solutions at 1:10 dilutions for CD11b-Pacific Blue (Bio-Rad/AbD Serotec Cat# MCA275PB, RRID:AB_566459), CD163-Biotin (Bio-Rad/AbD Serotec Cat# MCA342B, RRID:AB_2074559), CD68-PE (Bio-Rad/AbD Serotec Cat# MCA341PE, RRID:AB_324585), CD86-Alexafluor 647 (Bio-Rad/AbD Serotec Cat# MCA2874A647, RRID:AB_1719961), and CD45-PE/Alexafluor 750 (Bio-Rad/AbD Serotec Cat# MCA43P750, RRID:AB_10673436) and 1:1 dilution for CD80-FITC (LS Bio Cat#C188420–100) in PBS containing 0.5% FBS and 0.1% TritonX-100, referred to as flow buffer 2 (FB2), for 1 h at room temperature and protected from light.

Techniques: Flow Cytometry, Expressing

Journal: Journal of Neuroinflammation

Article Title: Sustained interleukin-10 delivery reduces inflammation and improves motor function after spinal cord injury

doi: 10.1186/s12974-019-1479-3

Figure Lengend Snippet: Percentages of M1 and M2 macrophages. The cells determined to be CD11b + CD45 HIGH macrophages were further analyzed for macrophage phenotype. Cells that were positive for the M1 markers CD68 or CD80 were characterized as “M1” macrophages and the CD68 − CD80 − macrophages were further analyzed with CD86 and CD163 to distinguish early stage“M2b” and later stage “M2c,” as shown in the schematic ( a ). The FMOs for CD68 and CD80 were used to set the gates and a representative sample of MCMs+IL-10 is shown for the CD68 vs CD80 ( b ). The FMOs for CD86 and CD163 were used to set the gates for distinguishing “M2b” and “M2c” cells and the CD68 − CD80 − “M2” macrophages were further analyzed for CD86 vs CD163 ( c ). Comparing across groups, MCMs+IL-10 had significantly less “M1” macrophages than the Controls, Local IL-10, and MCMs ( d ). MCMs+IL-10 also had significantly more “M2b” macrophages than the Controls, Local IL-10, and MCMs ( e ). * P < 0.05 (Tukey’s Test); error bars represent ± SEM; n = 3 for Controls and 4 for all other groups

Article Snippet: The cells were then incubated in 100 μL of primary antibody solutions at 1:10 dilutions for CD11b-Pacific Blue (Bio-Rad/AbD Serotec Cat# MCA275PB, RRID:AB_566459), CD163-Biotin (Bio-Rad/AbD Serotec Cat# MCA342B, RRID:AB_2074559), CD68-PE (Bio-Rad/AbD Serotec Cat# MCA341PE, RRID:AB_324585), CD86-Alexafluor 647 (Bio-Rad/AbD Serotec Cat# MCA2874A647, RRID:AB_1719961), and CD45-PE/Alexafluor 750 (Bio-Rad/AbD Serotec Cat# MCA43P750, RRID:AB_10673436) and 1:1 dilution for CD80-FITC (LS Bio Cat#C188420–100) in PBS containing 0.5% FBS and 0.1% TritonX-100, referred to as flow buffer 2 (FB2), for 1 h at room temperature and protected from light.

Techniques: